5BN2
Room Temperature Structure of Pichia pastoris aquaporin at 1.3 A
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | MAX II BEAMLINE I911-5 |
| Synchrotron site | MAX II |
| Beamline | I911-5 |
| Temperature [K] | 293 |
| Detector technology | CCD |
| Collection date | 2010-02-18 |
| Detector | MAR CCD 165 mm |
| Wavelength(s) | 0.9083 |
| Spacegroup name | I 4 |
| Unit cell lengths | 92.487, 92.487, 81.191 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 34.490 - 1.300 |
| R-factor | 0.11551 |
| Rwork | 0.114 |
| R-free | 0.14261 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3zoj |
| RMSD bond length | 0.022 |
| RMSD bond angle | 1.900 |
| Data reduction software | MOSFLM |
| Data scaling software | SCALA |
| Phasing software | PHASER |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 34.490 | 1.370 |
| High resolution limit [Å] | 1.300 | 1.300 |
| Rmerge | 0.059 | 0.499 |
| Number of reflections | 83459 | |
| <I/σ(I)> | 11.7 | 2.4 |
| Completeness [%] | 99.6 | 100 |
| Redundancy | 3.4 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293 | 26% PEG600, 100 MM TRIS (PH=8.0), 200 MM CACL2 |
