5BKN

Crystallographic structure of a cubic crystal form of STMV (84.5 degree rotation) grown from chloride

This is a non-PDB format compatible entry.

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	ALS BEAMLINE 8.3.1
Synchrotron site	ALS
Beamline	8.3.1
Temperature [K]	173
Detector technology	CCD
Collection date	2002-06-18
Detector	MAR CCD 165 mm
Wavelength(s)	1.0
Spacegroup name	P 2 3
Unit cell lengths	234.650, 234.650, 234.650
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	65.080 - 3.000
R-factor	0.2103
Rwork	0.208
R-free	0.25510
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	4oq8
RMSD bond length	0.005
RMSD bond angle	0.824
Data reduction software	d*TREK
Data scaling software	d*TREK
Phasing software	PHASER
Refinement software	PHENIX (1.19_4092)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	75.000	3.150
High resolution limit [Å]	3.000	3.000
Rmerge	0.128	0.229
Number of reflections	85962	7300
<I/σ(I)>	21.2	2.3
Completeness [%]	1.0
Redundancy	9.4
CC(1/2)	0.976	0.967

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	6	279	Crystals were grown by sitting drop vapor diffusion in Cryschem plates using .6 ml reservoirs. The drops were composed of equal volumes of a 5 mg/ml virus stock solution of 0.1 M phosphate at pH 6.5, and the reservoir solution, which was 8% NaCl w/v in 0.1 M phosphate at pH 6.0. Crystallization was at 4 degrees C.