5BJS
Apo ctPRC2 in an autoinhibited state
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 19-ID |
| Synchrotron site | APS |
| Beamline | 19-ID |
| Temperature [K] | 93 |
| Detector technology | PIXEL |
| Collection date | 2016-03-11 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.979 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 117.775, 137.897, 223.850 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.451 - 2.189 |
| R-factor | 0.1757 |
| Rwork | 0.173 |
| R-free | 0.22370 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5ch1 |
| RMSD bond length | 0.009 |
| RMSD bond angle | 0.884 |
| Data reduction software | HKL-3000 |
| Data scaling software | HKL-3000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.250 |
| High resolution limit [Å] | 2.190 | 2.190 |
| Rmerge | 0.126 | 1.540 |
| Number of reflections | 89897 | |
| <I/σ(I)> | 14.91 | 1.33 |
| Completeness [%] | 99.9 | 99.8 |
| Redundancy | 6.7 | 6.2 |
| CC(1/2) | 0.810 | 0.420 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 298 | 100 mM sodium malonate pH 7.0, 16% PEG 3350, 43 mM cyclohexyl-1-propylphosphocholine |
