5BJP
Crystal structure of the Corn RNA aptamer in complex with DFHO, iridium hexammine soak
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 5.0.2 |
| Synchrotron site | ALS |
| Beamline | 5.0.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-04-21 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.1050 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 36.642, 55.438, 98.676 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 49.340 - 2.510 |
| R-factor | 0.19547 |
| Rwork | 0.191 |
| R-free | 0.23902 |
| Structure solution method | SAD |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.878 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AutoSol |
| Refinement software | REFMAC (5.8.0103) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.500 |
| Rmerge | 0.130 |
| Number of reflections | 6468 |
| <I/σ(I)> | 23.7 |
| Completeness [%] | 98.8 |
| Redundancy | 12.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 294 | 32.5% PEG 550 MME, 0.1 M Tris-HCl pH 8.05 |
