5B80
Crystal Structure of Hyperthermophilic Thermotoga maritima L-Ketose-3-Epimerase with Cu2+
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 5C (4A) |
| Synchrotron site | PAL/PLS |
| Beamline | 5C (4A) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2016-06-03 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97940 |
| Spacegroup name | P 1 |
| Unit cell lengths | 50.282, 55.252, 58.763 |
| Unit cell angles | 107.04, 102.49, 91.80 |
Refinement procedure
| Resolution | 35.250 - 1.700 |
| R-factor | 0.1675 |
| Rwork | 0.166 |
| R-free | 0.18960 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5JTX |
| RMSD bond length | 0.019 |
| RMSD bond angle | 1.083 |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 61647 | |
| <I/σ(I)> | 14.64 | 3.63 |
| Completeness [%] | 95.9 | 94.4 |
| Redundancy | 3.3 | 3.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6 | 293 | 100mM MES, 5%(w/w) PEG1000, 20%(v/v) PEG200 |
