5B7D
OxyR2 E204G mutant regulatory domain from Vibrio vulnificus (sulfate-bound)
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PAL/PLS BEAMLINE 7A (6B, 6C1) |
| Synchrotron site | PAL/PLS |
| Beamline | 7A (6B, 6C1) |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-03-13 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.97933 |
| Spacegroup name | C 2 2 21 |
| Unit cell lengths | 72.705, 137.238, 96.655 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 19.756 - 1.520 |
| R-factor | 0.1586 |
| Rwork | 0.157 |
| R-free | 0.18310 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5B6U |
| RMSD bond length | 0.008 |
| RMSD bond angle | 0.964 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((1.11.1_2575: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.550 |
| High resolution limit [Å] | 1.520 | 1.520 |
| Rmerge | 0.070 | 0.360 |
| Number of reflections | 74251 | |
| <I/σ(I)> | 31.39 | 3.8 |
| Completeness [%] | 99.7 | 99.1 |
| Redundancy | 10.8 | 6.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.6 | 287 | 0.2M ammonium sulfate, 0.1M sodium citrate (pH 5.6), 11% PEG 4000, 2mM TCEP |
