5B75
Crystal structure of MOZ double PHD finger in complex with histone H3 butyrylation at K14
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SSRF BEAMLINE BL17U |
| Synchrotron site | SSRF |
| Beamline | BL17U |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-01-03 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 0.97920 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 47.677, 48.122, 76.191 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.869 - 1.704 |
| R-factor | 0.1708 |
| Rwork | 0.170 |
| R-free | 0.19500 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4llb |
| RMSD bond length | 0.010 |
| RMSD bond angle | 1.000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 29.900 | 1.730 |
| High resolution limit [Å] | 1.700 | 1.700 |
| Number of reflections | 19643 | |
| <I/σ(I)> | 22.7 | |
| Completeness [%] | 99.8 | |
| Redundancy | 4.5 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 290 | polyethylene glycol 4000, lithium sulfate, Tris |
