5B6T
Catalytic domain of Coprinopsis cinerea GH62 alpha-L-arabinofuranosidase complexed with Pb
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NE3A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-12-13 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 0.95064 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 47.854, 77.765, 74.458 |
| Unit cell angles | 90.00, 93.01, 90.00 |
Refinement procedure
| Resolution | 27.630 - 1.480 |
| R-factor | 0.15162 |
| Rwork | 0.150 |
| R-free | 0.17434 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4n4b |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.507 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0049) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 27.630 | 1.530 |
| High resolution limit [Å] | 1.480 | 1.480 |
| Rmerge | 0.073 | 0.441 |
| Number of reflections | 321802 | |
| <I/σ(I)> | 24.5 | 3.8 |
| Completeness [%] | 99.4 | 97.3 |
| Redundancy | 3.6 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 6.5 | 293 | MES buffer, NaBr, PEG 20000 |
