5B6Q

Crystal structure of monomeric cytochrome c5 from Shewanella violacea

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	ROTATING ANODE
Source details	RIGAKU MICROMAX-007
Temperature [K]	100
Detector technology	IMAGE PLATE
Collection date	2015-03-02
Detector	RIGAKU RAXIS IV++
Wavelength(s)	1.5418
Spacegroup name	P 21 21 21
Unit cell lengths	26.588, 50.075, 97.609
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	34.951 - 1.780
R-factor	0.2164
Rwork	0.214
R-free	0.23750
Structure solution method	MOLECULAR REPLACEMENT
RMSD bond length	0.008
RMSD bond angle	1.693
Data reduction software	CrystalClear
Data scaling software	d*TREK
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	34.951
High resolution limit [Å]	1.780
Number of reflections	12711
<I/σ(I)>	16.1
Completeness [%]	96.7
Redundancy	6.12

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION		277	0.1 M imidazole, 0.2 M NaCl, 0.4 M NaH2PO4, 1.8 M K2HPO4