5B6E

Crystal Structure of cytidine monophosphate hydroxymethylase MilA with hmCMP

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SSRF BEAMLINE BL17U
Synchrotron site	SSRF
Beamline	BL17U
Temperature [K]	100
Detector technology	CCD
Collection date	2014-12-01
Detector	ADSC QUANTUM 315r
Wavelength(s)	0.97912
Spacegroup name	P 32 2 1
Unit cell lengths	109.323, 109.323, 112.867
Unit cell angles	90.00, 90.00, 120.00

Refinement procedure

Resolution	49.240 - 1.800
R-factor	0.1881
Rwork	0.187
R-free	0.20750
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5jnh
RMSD bond length	0.005
RMSD bond angle	0.984
Data scaling software	HKL-3000
Phasing software	PHASER
Refinement software	REFMAC (5.7.0032)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.860
High resolution limit [Å]	1.800	1.800
Number of reflections	68810
<I/σ(I)>	19.6
Completeness [%]	100.0
Redundancy	11.2

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	EVAPORATION	8.5	289	0.1 M sodium cacodylate trihydrate(pH 6.5), 1.4 M sodium acetate trihydrate