5B68

Crystal structure of apo amylomaltase from Corynebacterium glutamicum

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PAL/PLS BEAMLINE 7A (6B, 6C1)
Synchrotron site	PAL/PLS
Beamline	7A (6B, 6C1)
Temperature [K]	100
Detector technology	CCD
Collection date	2016-05-02
Detector	ADSC QUANTUM 270
Wavelength(s)	0.97934
Spacegroup name	P 21 21 21
Unit cell lengths	73.044, 83.251, 124.821
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 1.700
R-factor	0.1994
Rwork	0.181
R-free	0.22200
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5jjh
RMSD bond length	0.018
RMSD bond angle	1.863
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	REFMAC (5.8.0135)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000		1.730
High resolution limit [Å]	1.700	4.610	1.700
Rmerge	0.068	0.055	0.298
Number of reflections	81053
<I/σ(I)>	14
Completeness [%]	96.2	96.8	90.7
Redundancy	5	6.1	2.9

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6.5	295	Ammonium sulfate, Bis-tris