5B66

Crystal structure analysis of Photosystem II complex

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	CCD
Collection date	2015-07-22
Detector	RAYONIX MX300HE
Wavelength(s)	0.9
Spacegroup name	P 21 21 21
Unit cell lengths	121.465, 228.176, 286.422
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	19.990 - 1.850
R-factor	0.16398
Rwork	0.162
R-free	0.20073
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3wu2
RMSD bond length	0.016
RMSD bond angle	2.321
Data reduction software	XDS
Data scaling software	SCALA (3.3.22)
Phasing software	MOLREP (11.2.08)
Refinement software	REFMAC (5.6.0117)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	49.000	1.950
High resolution limit [Å]	1.850	1.850
Rmerge	0.120	1.500
Number of reflections	671082
<I/σ(I)>	20.5	1.9
Completeness [%]	100.0	100
Redundancy	15.5	15.4
CC(1/2)	0.999

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	MICROBATCH	6.1	285	Mes, NaCl, CaCl2, MgSO4, PEG 1450