5B5Y
Crystal structure of PtLCIB4, a homolog of the limiting CO2-inducible protein LCIB
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | AUSTRALIAN SYNCHROTRON BEAMLINE MX2 |
| Synchrotron site | Australian Synchrotron |
| Beamline | MX2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-10-01 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 |
| Unit cell lengths | 47.910, 48.040, 59.980 |
| Unit cell angles | 87.96, 102.79, 93.31 |
Refinement procedure
| Resolution | 36.658 - 1.750 |
| R-factor | 0.1764 |
| Rwork | 0.175 |
| R-free | 0.20190 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5b5x |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.148 |
| Data reduction software | SCALA |
| Data scaling software | SCALA |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 36.660 | 1.840 |
| High resolution limit [Å] | 1.750 | 1.750 |
| Number of reflections | 52517 | |
| <I/σ(I)> | 9.9 | |
| Completeness [%] | 93.5 | |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8 | 293.15 | 200 mM sodium acetate trihydrate, 100 mM Tris, 27% w/v PEG4000 |
