5B5I

The crystal structure of a crustacean hyperglycemic hormone precursor from the kuruma prawn

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-5A
Synchrotron site	Photon Factory
Beamline	BL-5A
Temperature [K]	95
Detector technology	CCD
Collection date	2009-04-25
Detector	ADSC QUANTUM 315
Wavelength(s)	0.9792
Spacegroup name	P 21 21 21
Unit cell lengths	38.679, 55.793, 55.793
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	7.944 - 1.599
R-factor	0.1902
Rwork	0.189
R-free	0.20570
RMSD bond length	0.007
RMSD bond angle	0.944
Data reduction software	SCALEPACK
Data scaling software	HKL-2000
Phasing software	MOLREP
Refinement software	PHENIX ((1.10.1_2155: ???))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.630
High resolution limit [Å]	1.600	1.600
Number of reflections	16354
<I/σ(I)>	38.72
Completeness [%]	99.9
Redundancy	14.1

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP		293	0.8-1.0 M NaCl, 50 mM potassium sodium tartrate, 50 mM tartaric acid, 5% ethanol, 25% 1,2-ethanediol