5B53
Crystal structure of hydrogen sulfide-producing enzyme (Fn1055) from Fusobacterium nucleatum
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2011-02-27 |
| Detector | ADSC QUANTUM 210r |
| Wavelength(s) | 1.0000 |
| Spacegroup name | P 43 2 2 |
| Unit cell lengths | 58.793, 58.793, 205.053 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.640 - 2.910 |
| R-factor | 0.17502 |
| Rwork | 0.171 |
| R-free | 0.26555 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1o58 |
| RMSD bond length | 0.018 |
| RMSD bond angle | 2.150 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.7.0029) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 33.640 | 2.950 |
| High resolution limit [Å] | 2.900 | 2.900 |
| Rmerge | 0.137 | 0.300 |
| Number of reflections | 8510 | |
| <I/σ(I)> | 17.4 | 8.5 |
| Completeness [%] | 98.5 | 98.6 |
| Redundancy | 3.8 | 3.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 293 | 4% (w/v) PEG 8000, 0.1M Hepes pH 7.5 |
