5B4N
Structure analysis of function associated loop mutant of substrate recognition domain of Fbs1 ubiquitin ligase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2013-06-20 |
| Detector | MAR scanner 300 mm plate |
| Wavelength(s) | 0.9 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 44.541, 96.203, 44.681 |
| Unit cell angles | 90.00, 101.90, 90.00 |
Refinement procedure
| Resolution | 39.800 - 2.300 |
| R-factor | 0.18882 |
| Rwork | 0.185 |
| R-free | 0.26571 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1umh |
| RMSD bond length | 0.016 |
| RMSD bond angle | 1.764 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.340 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Rmerge | 0.099 | 0.436 |
| Number of reflections | 16552 | |
| <I/σ(I)> | 22.6 | |
| Completeness [%] | 99.9 | |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.15 | 20%PEG 3,350, 1%Tryptone and 0.05M HEPES-Na (pH 7.0) |
