5B3V
Crystal structure of biliverdin reductase in complex with biliverdin and NADP+ from Synechocystis sp. PCC 6803
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL44XU |
Synchrotron site | SPring-8 |
Beamline | BL44XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2014-12-08 |
Detector | RAYONIX MX300HE |
Wavelength(s) | 0.900 |
Spacegroup name | P 1 21 1 |
Unit cell lengths | 55.500, 100.413, 138.028 |
Unit cell angles | 90.00, 95.53, 90.00 |
Refinement procedure
Resolution | 49.624 - 2.594 |
R-factor | 0.2103 |
Rwork | 0.208 |
R-free | 0.25760 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 5b3t |
RMSD bond length | 0.005 |
RMSD bond angle | 1.761 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | PHENIX |
Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 2.640 |
High resolution limit [Å] | 2.594 | 2.600 |
Rmerge | 0.094 | 0.473 |
Number of reflections | 46736 | |
<I/σ(I)> | 7.4 | 19.83 |
Completeness [%] | 99.9 | 100 |
Redundancy | 4.2 | 4.2 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 293 | PEG4000, Trizma (pH8.2), magnesium chloride, barium chloride |