5B3R
Crystal structure of metallo-beta-lactamase IMP-18 from Pseudomonas aeruginosa
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-11-15 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.00 |
| Spacegroup name | P 2 2 21 |
| Unit cell lengths | 78.550, 87.380, 105.750 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 30.000 - 2.000 |
| R-factor | 0.20229 |
| Rwork | 0.201 |
| R-free | 0.23199 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.317 |
| Data reduction software | HKL-2000 |
| Data scaling software | SCALEPACK |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 36.820 |
| High resolution limit [Å] | 1.970 |
| Number of reflections | 50965 |
| <I/σ(I)> | 6.6 |
| Completeness [%] | 98.7 |
| Redundancy | 14.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 283 | sodium citrate, polyethylene glycol 4000, ethylene glycol, Strontium chloride |
