5B37
Crystal structure of L-tryptophan dehydrogenase from Nostoc punctiforme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NE3A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NE3A |
| Temperature [K] | 93 |
| Detector technology | CCD |
| Collection date | 2015-06-12 |
| Detector | ADSC QUANTUM 270 |
| Wavelength(s) | 1.00000 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 63.713, 337.766, 63.646 |
| Unit cell angles | 90.00, 120.03, 90.00 |
Refinement procedure
| Resolution | 50.000 - 3.400 |
| R-factor | 0.20498 |
| Rwork | 0.203 |
| R-free | 0.25418 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3vpx |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.177 |
| Data reduction software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0135) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 3.460 |
| High resolution limit [Å] | 3.400 | 3.400 |
| Rmerge | 0.088 | |
| Number of reflections | 88938 | |
| <I/σ(I)> | 8.3 | |
| Completeness [%] | 99.1 | |
| Redundancy | 2.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7.5 | 277 | HEPES-NaOH (pH 7.5), polyethylene glycol 8000, ethylene glycol |
