5B28
The 0.90A structure of human FABP3 F16V mutant complexed with palmitic acid
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-07-09 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.800 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 54.555, 70.094, 33.785 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 43.050 - 0.900 |
| R-factor | 0.11323 |
| Rwork | 0.113 |
| R-free | 0.12624 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2hmb |
| RMSD bond length | 0.017 |
| RMSD bond angle | 1.586 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.5.0110) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 43.050 | 0.920 |
| High resolution limit [Å] | 0.900 | 0.900 |
| Rmerge | 0.052 | 0.412 |
| Number of reflections | 95381 | |
| <I/σ(I)> | 10.1 | 3.6 |
| Completeness [%] | 98.6 | 99.8 |
| Redundancy | 11.7 | 8.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8 | 293 | 0.1M Tris-HCl (pH8.0), 55% (v/v) PEG 400 |
