5B22
Dimer structure of murine Nectin-3 D1D2
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-1A |
| Synchrotron site | Photon Factory |
| Beamline | BL-1A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-10-23 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.1000 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 151.650, 72.880, 81.930 |
| Unit cell angles | 90.00, 116.36, 90.00 |
Refinement procedure
| Resolution | 29.770 - 2.580 |
| R-factor | 0.2321 |
| Rwork | 0.229 |
| R-free | 0.28840 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 4fom |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.268 |
| Data reduction software | iMOSFLM |
| Data scaling software | SCALA |
| Refinement software | PHENIX ((1.10.1_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | |
| High resolution limit [Å] | 2.580 | 2.580 |
| Rmerge | 0.110 | |
| Number of reflections | 25302 | |
| <I/σ(I)> | 6.5 | |
| Completeness [%] | 99.7 | 99.8 |
| Redundancy | 2.9 | 3.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293.15 | 22% PEG 3350, 100mM Imidazole pH7.0, 100mM Ammmonium citrate tribasic |
