5B21
Dimer structure of murine Nectin-1 D1
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-07-08 |
| Detector | BRUKER SMART 6500 |
| Wavelength(s) | 0.90000 |
| Spacegroup name | P 32 2 1 |
| Unit cell lengths | 78.320, 78.320, 70.840 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 39.160 - 2.240 |
| R-factor | 0.206 |
| Rwork | 0.204 |
| R-free | 0.25800 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3alp |
| RMSD bond length | 0.002 |
| RMSD bond angle | 0.663 |
| Data reduction software | iMOSFLM |
| Data scaling software | iMOSFLM |
| Phasing software | MOLREP |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 39.160 | 2.318 |
| High resolution limit [Å] | 2.240 | 2.238 |
| Rmerge | 0.162 | |
| Number of reflections | 12470 | |
| <I/σ(I)> | 7.3 | |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 7 | 7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 293 | 40% PEG3350, 70 mM Na-citrate pH 5.0 |
