5B1L

The mouse nucleosome structure containing H3t

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE BL-1A
Synchrotron site	Photon Factory
Beamline	BL-1A
Temperature [K]	100
Detector technology	PIXEL
Collection date	2014-02-03
Detector	DECTRIS PILATUS 2M-F
Wavelength(s)	1.1
Spacegroup name	P 21 21 21
Unit cell lengths	98.968, 107.425, 167.332
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	41.833 - 2.350
R-factor	0.1993
Rwork	0.197
R-free	0.24290
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	2cv5
RMSD bond length	0.010
RMSD bond angle	1.261
Data scaling software	HKL-2000 (704y)
Phasing software	PHASER (2.5.6)
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall	Inner shell	Outer shell
Low resolution limit [Å]	50.000	50.000	2.430
High resolution limit [Å]	2.350	5.060	2.350
Rmerge	0.086	0.058	0.454
Rmeas	0.094	0.063	0.493
Rpim	0.036	0.025	0.190
Total number of observations	496153
Number of reflections	75240
<I/σ(I)>	5.9
Completeness [%]	100.0	99.9	100
Redundancy	6.6	6.3	6.7
CC(1/2)		0.998	0.897

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP	6	293	potassium cacodylate, potassium chloride, manganese chloride