5B1F
Crystal structure of hen egg-white lysozyme
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | FREE ELECTRON LASER |
| Source details | SACLA BEAMLINE BL3 |
| Synchrotron site | SACLA |
| Beamline | BL3 |
| Temperature [K] | 300 |
| Detector technology | CCD |
| Collection date | 2015-02-01 |
| Detector | MPCCD |
| Wavelength(s) | 1.77 |
| Spacegroup name | P 43 21 2 |
| Unit cell lengths | 79.100, 79.100, 38.000 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 25.014 - 2.300 |
| R-factor | 0.1753 |
| Rwork | 0.173 |
| R-free | 0.21470 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3wul |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.135 |
| Refinement software | PHENIX (1.8.4_1496) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 30.000 | 2.340 |
| High resolution limit [Å] | 2.300 | 2.300 |
| Number of reflections | 5727 | |
| <I/σ(I)> | 11.1 | 10.4 |
| Completeness [%] | 100.0 | 100 |
| Redundancy | 453 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | BATCH MODE | 293 | NaCl |
