5B1C
Crystal structure of DEN4 ED3 mutant with L387I
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 95 |
| Detector technology | PIXEL |
| Collection date | 2014-11-28 |
| Detector | DECTRIS PILATUS3 6M |
| Wavelength(s) | 0.9800 |
| Spacegroup name | C 2 2 2 |
| Unit cell lengths | 125.090, 130.863, 64.585 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 33.839 - 2.003 |
| R-factor | 0.2166 |
| Rwork | 0.215 |
| R-free | 0.23970 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3we1 |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.176 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.070 |
| High resolution limit [Å] | 2.000 | 2.000 |
| Rmerge | 0.872 | |
| Number of reflections | 35906 | |
| <I/σ(I)> | 48.82 | 5.1 |
| Completeness [%] | 99.8 | 100 |
| Redundancy | 7 | 7.1 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 8.5 | 277 | PEG 4000, Ammonium Sulfate, Tris. HCl, Dioxane |
