5B0V
Crystal Structure of Marburg virus VP40 Dimer
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | APS BEAMLINE 23-ID-B |
| Synchrotron site | APS |
| Beamline | 23-ID-B |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-08-19 |
| Detector | MARMOSAIC 300 mm CCD |
| Wavelength(s) | 0.979, 0.980 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 95.810, 107.310, 127.650 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 29.670 - 2.810 |
| R-factor | 0.20459 |
| Rwork | 0.202 |
| R-free | 0.25686 |
| Structure solution method | MAD |
| RMSD bond length | 0.011 |
| RMSD bond angle | 1.546 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | Auto-Rickshaw |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.810 |
| Number of reflections | 25037 |
| <I/σ(I)> | 6.9 |
| Completeness [%] | 99.5 |
| Redundancy | 4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 295 | 17.5% ethanol, 75mM NaCl, 10mM CHAPS, 100mM Tris-HCl, pH 7.7 |
