5B0R
Beta-1,2-Mannobiose phosphorylase from Listeria innocua - beta-1,2-mannobiose complex
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-06-22 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.000 |
| Spacegroup name | P 31 2 1 |
| Unit cell lengths | 145.773, 145.773, 105.774 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 32.930 - 1.800 |
| R-factor | 0.16624 |
| Rwork | 0.165 |
| R-free | 0.19322 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 1vkd |
| RMSD bond length | 0.029 |
| RMSD bond angle | 2.480 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 1.830 |
| High resolution limit [Å] | 1.800 | 1.800 |
| Rmerge | 0.054 | 0.351 |
| Number of reflections | 119774 | |
| <I/σ(I)> | 55.5 | 6.3 |
| Completeness [%] | 99.9 | 100 |
| Redundancy | 11.2 | 11 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 6.6 | 293 | 1.8 M (NH4)2SO4, 10 mM CoCl2, 0.1 M MES-NaOH |
