5B0P
Beta-1,2-Mannobiose phosphorylase from Listeria innocua - glycerol complex
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
Synchrotron site | Photon Factory |
Beamline | AR-NW12A |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2013-10-27 |
Detector | ADSC QUANTUM 210 |
Wavelength(s) | 1.000 |
Spacegroup name | P 31 2 1 |
Unit cell lengths | 146.559, 146.559, 106.324 |
Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
Resolution | 34.140 - 1.900 |
R-factor | 0.16622 |
Rwork | 0.165 |
R-free | 0.19698 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 1vkd |
RMSD bond length | 0.027 |
RMSD bond angle | 2.205 |
Data reduction software | HKL-2000 |
Data scaling software | HKL-2000 |
Phasing software | MOLREP |
Refinement software | REFMAC (5.8.0107) |
Data quality characteristics
Overall | Outer shell | |
Low resolution limit [Å] | 50.000 | 1.930 |
High resolution limit [Å] | 1.900 | 1.900 |
Rmerge | 0.057 | 0.468 |
Number of reflections | 103595 | |
<I/σ(I)> | 50.1 | 5.7 |
Completeness [%] | 100.0 | 100 |
Redundancy | 9.4 | 9.4 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | VAPOR DIFFUSION, SITTING DROP | 6.6 | 293 | 1.8 M (NH4)2SO4, 10 mM CoCl2, 0.1 M MES-NaOH |