5B02

Structure of the prenyltransferase MoeN5 with a fusion protein tag of Sso7d

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	NSRRC BEAMLINE BL13B1
Synchrotron site	NSRRC
Beamline	BL13B1
Temperature [K]	100
Detector technology	CCD
Collection date	2015-04-15
Detector	ADSC QUANTUM 315r
Wavelength(s)	1.0
Spacegroup name	C 2 2 2
Unit cell lengths	137.879, 217.441, 104.637
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	25.000 - 2.210
Rwork	0.176
R-free	0.21800
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	5b00 1bf4
Data reduction software	HKL-2000
Data scaling software	HKL-2000
Phasing software	CNS
Refinement software	CNS

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	25.000	2.290
High resolution limit [Å]	2.210	2.210
Rmerge	0.084	0.489
Number of reflections	78788
<I/σ(I)>	17.4	2.4
Completeness [%]	99.9	99.9
Redundancy	5.8	5.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.1	298	0.2M Li3-citrate, 0.3M NaCl, 25% PEG 3350