5B00
Structure of the prenyltransferase MoeN5 in complex with geranyl pyrophosphate
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-01-14 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 206.850, 58.547, 92.678 |
| Unit cell angles | 90.00, 91.30, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.950 |
| Rwork | 0.287 |
| R-free | 0.33700 |
| Structure solution method | SAD |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | CNS |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 25.000 | 3.060 |
| High resolution limit [Å] | 2.950 | 2.950 |
| Rmerge | 0.087 | 0.453 |
| Number of reflections | 23648 | |
| <I/σ(I)> | 19.9 | 3.5 |
| Completeness [%] | 99.0 | 93.4 |
| Redundancy | 4.5 | 4.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 9.1 | 298 | 0.2 M Na2HPO4, 16% PEG 3350 |
