5AZW
Crystal structure of p24beta1 GOLD domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE AR-NW12A |
| Synchrotron site | Photon Factory |
| Beamline | AR-NW12A |
| Temperature [K] | 95 |
| Detector technology | CCD |
| Collection date | 2012-05-31 |
| Detector | ADSC QUANTUM 210 |
| Wavelength(s) | 1.0 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 97.772, 35.664, 62.312 |
| Unit cell angles | 90.00, 129.22, 90.00 |
Refinement procedure
| Resolution | 24.137 - 1.500 |
| R-factor | 0.2214 |
| Rwork | 0.221 |
| R-free | 0.23310 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 0.969 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((1.10_2155: ???)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 100.000 | 1.530 |
| High resolution limit [Å] | 1.500 | 1.500 |
| Rmerge | 0.346 | |
| Number of reflections | 26802 | |
| <I/σ(I)> | 79 | 6.8 |
| Completeness [%] | 99.3 | 99.3 |
| Redundancy | 7.3 | 7.3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 8.5 | 293 | 0.1 M Tris-HCl (pH 8.5), 0.2 M sodium chloride and 25 % (w/v) PEG 3,350 |
