5AZD
Crystal structure of thermophilic rhodopsin.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-1A |
| Synchrotron site | Photon Factory |
| Beamline | BL-1A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2013-10-30 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.1 |
| Spacegroup name | P 1 |
| Unit cell lengths | 63.186, 67.693, 73.045 |
| Unit cell angles | 116.48, 114.14, 90.82 |
Refinement procedure
| Resolution | 46.519 - 2.800 |
| R-factor | 0.2311 |
| Rwork | 0.228 |
| R-free | 0.27770 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3ddl |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.737 |
| Phasing software | PHASER |
| Refinement software | PHENIX (1.9_1692) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 46.520 | 2.896 |
| High resolution limit [Å] | 2.800 | 2.796 |
| Rmerge | 0.119 | 0.454 |
| Number of reflections | 22122 | |
| <I/σ(I)> | 6.26 | 1.77 |
| Completeness [%] | 93.2 | 89.08 |
| Redundancy | 1.8 | 1.8 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | LIPIDIC CUBIC PHASE | 3.6 | 296 | 100mM sodium citrate pH3.6, 34% PEG 400 (v/v) |
