5AZ3

Crystal structure of heme binding protein HmuT

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL44XU
Synchrotron site	SPring-8
Beamline	BL44XU
Temperature [K]	100
Detector technology	CCD
Collection date	2014-02-17
Detector	MARMOSAIC 300 mm CCD
Wavelength(s)	0.90
Spacegroup name	P 41 21 2
Unit cell lengths	73.040, 73.040, 147.320
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	35.447 - 1.423
R-factor	0.1689
Rwork	0.168
R-free	0.18150
Structure solution method	MOLECULAR REPLACEMENT
Starting model (for MR)	3md9
RMSD bond length	0.007
RMSD bond angle	1.116
Data scaling software	SCALA
Phasing software	PHASER
Refinement software	PHENIX ((phenix.refine: 1.8.4_1496))

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	35.590	1.500
High resolution limit [Å]	1.420	1.420
Rmerge	0.075	0.317
Number of reflections	75260
<I/σ(I)>	19.9	6.9
Completeness [%]	99.7	99.4
Redundancy	13.7	12.6

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, SITTING DROP	8.5	293	2.1 M ammonium sulfate, 0.2 M potassium thiocyanate