5AYO

Crystal structure of a bacterial homologue of iron transporter ferroportin in inward-facing state

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL32XU
Synchrotron site	SPring-8
Beamline	BL32XU
Temperature [K]	100
Detector technology	CCD
Collection date	2014-10-26
Detector	RAYONIX MX225HE
Wavelength(s)	1.2820
Spacegroup name	P 21 21 21
Unit cell lengths	63.530, 70.680, 101.790
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	42.857 - 3.300
R-factor	0.2403
Rwork	0.235
R-free	0.28460
RMSD bond length	0.002
RMSD bond angle	0.642
Data scaling software	XSCALE
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	42.900
High resolution limit [Å]	3.300
Number of reflections	13103
<I/σ(I)>	7.8
Completeness [%]	98.5
Redundancy	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE	4	293	100 mM Na-acetate, pH 4.0, 200-300 mM K-formate, 10 mM ZnSO4, 10-20 mM ZnCl2, 27-30% PEG550MME