5AYM

Crystal structure of a bacterial homologue of iron transporter ferroportin in outward-facing state with soaked iron

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	SPRING-8 BEAMLINE BL32XU
Synchrotron site	SPring-8
Beamline	BL32XU
Temperature [K]	100
Detector technology	CCD
Collection date	2014-07-20
Detector	RAYONIX MX225HE
Wavelength(s)	1.450
Spacegroup name	P 21 21 21
Unit cell lengths	57.080, 86.170, 96.710
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	49.157 - 3.000
R-factor	0.2283
Rwork	0.224
R-free	0.26890
RMSD bond length	0.002
RMSD bond angle	0.604
Data scaling software	XDS
Phasing software	PHASER
Refinement software	PHENIX (1.9_1692)

Data quality characteristics

	Overall
Low resolution limit [Å]	49.200
High resolution limit [Å]	3.000
Number of reflections	18454
<I/σ(I)>	10.4
Completeness [%]	100.0
Redundancy	7.5

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	LIPIDIC CUBIC PHASE	7.5	293	100 mM Tris, pH 7.5, 50-200 mM K-formate, 50-100 mM (NH4)2HPO4, and 26-32% PEG550MME, 5 mM FeSO4 soaked