5AYL
Crystal structure of ERdj5 form II
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-1A |
| Synchrotron site | Photon Factory |
| Beamline | BL-1A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-01-22 |
| Detector | DECTRIS PILATUS 2M-F |
| Wavelength(s) | 1.10 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 75.981, 75.564, 79.237 |
| Unit cell angles | 90.00, 99.41, 90.00 |
Refinement procedure
| Resolution | 39.085 - 2.400 |
| R-factor | 0.1916 |
| Rwork | 0.190 |
| R-free | 0.23020 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3apo |
| RMSD bond length | 0.003 |
| RMSD bond angle | 0.744 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.460 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.688 | |
| Number of reflections | 68376 | |
| <I/σ(I)> | 9.1 | 1.2 |
| Completeness [%] | 98.0 | 82.1 |
| Redundancy | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 7.5 | 293 | PEG8000, cystin |
