5AY7
A psychrophilic glycoside hydrolase family 10 endo-beta-1,4-xylanase
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | NSRRC BEAMLINE BL13B1 |
| Synchrotron site | NSRRC |
| Beamline | BL13B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2015-03-19 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 59.434, 78.346, 89.296 |
| Unit cell angles | 90.00, 103.15, 90.00 |
Refinement procedure
| Resolution | 25.000 - 2.150 |
| R-factor | 0.1948 |
| Rwork | 0.192 |
| R-free | 0.25738 |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.689 |
| Data reduction software | REFMAC |
| Data scaling software | HKL-2000 |
| Phasing software | Coot |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 25.000 |
| High resolution limit [Å] | 2.150 |
| Number of reflections | 42567 |
| <I/σ(I)> | 21.9 |
| Completeness [%] | 98.4 |
| Redundancy | 3.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | EVAPORATION | 8.5 | 298 | 0.1M Tris, pH 8.5, 0.2M Li2SO4, 30% (w/v) PEG 4000 |
