5AY6
32 kDa Fragment of the Flagellar hook protein FlgE from Caulobacter crescentus
Experimental procedure
| Experimental method | MAD |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2012-10-11 |
| Detector | MAR scanner 300 mm plate |
| Wavelength(s) | 0.9, 0.9791 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 55.117, 61.580, 103.347 |
| Unit cell angles | 90.00, 91.92, 90.00 |
Refinement procedure
| Resolution | 19.785 - 1.839 |
| R-factor | 0.1785 |
| Rwork | 0.176 |
| R-free | 0.22160 |
| Structure solution method | SAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.931 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.8.4_1496)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 19.785 | 1.870 |
| High resolution limit [Å] | 1.839 | 1.840 |
| Number of reflections | 53991 | |
| <I/σ(I)> | 4 | |
| Completeness [%] | 89.6 | 85.2 |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 9 | 293 | 0.1 M TAPS, 1 M Potassium citrate, 5% 1,5-pentanediol |
