5AY3
Crystal structure of RNA duplex containing C-C base pairs
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | PHOTON FACTORY BEAMLINE BL-17A |
| Synchrotron site | Photon Factory |
| Beamline | BL-17A |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-11-25 |
| Detector | DECTRIS PILATUS 6M |
| Wavelength(s) | 0.98 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 27.160, 44.460, 27.370 |
| Unit cell angles | 90.00, 109.43, 90.00 |
Refinement procedure
| Resolution | 25.800 - 1.200 |
| Rwork | 0.184 |
| R-free | 0.19850 |
| Structure solution method | SAD |
| RMSD bond length | 0.004 |
| RMSD bond angle | 0.910 |
| Data scaling software | XSCALE |
| Phasing software | PHENIX |
| Refinement software | CNS (1.3) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 25.800 | 25.800 | 1.230 |
| High resolution limit [Å] | 1.200 | 5.370 | 1.200 |
| Rmerge | 0.020 | 0.188 | |
| Rmeas | 0.024 | 0.227 | |
| Number of reflections | 19127 | 230 | 1405 |
| <I/σ(I)> | 22.16 | 50.64 | 5.41 |
| Completeness [%] | 99.3 | 97.9 | 98.5 |
| Redundancy | 3.2 | 3 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 7 | 293 | MPD, Spermine, Lithium Nitrate, MOPS |
