5AXV
Crystal Structure of Cypovirus Polyhedra R13K Mutant
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL32XU |
| Synchrotron site | SPring-8 |
| Beamline | BL32XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2011-07-02 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 1 |
| Spacegroup name | I 2 3 |
| Unit cell lengths | 103.381, 103.381, 103.381 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 36.550 - 2.040 |
| R-factor | 0.152 |
| Rwork | 0.149 |
| R-free | 0.20600 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2oh6 |
| RMSD bond length | 0.014 |
| RMSD bond angle | 1.653 |
| Data scaling software | HKL-2000 |
| Phasing software | MOLREP |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 40.000 | 2.080 |
| High resolution limit [Å] | 2.040 | 2.040 |
| Number of reflections | 11308 | |
| <I/σ(I)> | 6.15 | 2.2 |
| Completeness [%] | 93.3 | 97.3 |
| Redundancy | 2.8 | 2.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | IN CELL | 7 | 300 | IN VIVO CRYSTALLIZATION IN THE CYTOPLASM OF THE CELL, PH 7.0, TEMPERATURE 300K |
