5AXC

Crystal structure of mouse SAHH complexed with 3'-keto aristeromycin

Experimental procedure

Experimental method	SINGLE WAVELENGTH
Source type	SYNCHROTRON
Source details	PHOTON FACTORY BEAMLINE AR-NE3A
Synchrotron site	Photon Factory
Beamline	AR-NE3A
Temperature [K]	100
Detector technology	CCD
Collection date	2010-03-13
Detector	ADSC QUANTUM 270
Wavelength(s)	1.0
Spacegroup name	I 2 2 2
Unit cell lengths	98.202, 102.881, 174.827
Unit cell angles	90.00, 90.00, 90.00

Refinement procedure

Resolution	50.000 - 1.550
R-factor	0.18099
Rwork	0.179
R-free	0.21018
RMSD bond length	0.016
RMSD bond angle	1.722
Refinement software	REFMAC (5.5.0110)

Data quality characteristics

	Overall	Outer shell
Low resolution limit [Å]	50.000	1.580
High resolution limit [Å]	1.550	1.550
Rmerge		0.513
Number of reflections	125615
<I/σ(I)>	44.7	2.5
Completeness [%]	98.4	90.8
Redundancy	5.8	4

Crystallization Conditions

crystal ID	method	pH	temperature	details
1	VAPOR DIFFUSION, HANGING DROP		293	PEG 3350