5AX7
yeast pyruvyltransferase Pvg1p
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-11-20 |
| Detector | ADSC QUANTUM 315r |
| Wavelength(s) | 1.3 |
| Spacegroup name | P 31 |
| Unit cell lengths | 85.529, 85.529, 93.611 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 10.990 - 2.460 |
| R-factor | 0.16814 |
| Rwork | 0.167 |
| R-free | 0.18335 |
| Structure solution method | SAD |
| RMSD bond length | 0.005 |
| RMSD bond angle | 0.959 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 10.990 |
| High resolution limit [Å] | 2.460 |
| Number of reflections | 55667 |
| <I/σ(I)> | 25.14 |
| Completeness [%] | 99.8 |
| Redundancy | 5.77 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 293.4 | 200 mM magnesium choloride, 10 mM zinc chloride, 26% (w/v) Poly(acrylic acid sodium salt) 5100 in 0.1 M HEPES (pH 7.5) |
