5AX6
The crystal structure of CofB, the minor pilin subunit of CFA/III from human enterotoxigenic Escherichia coli.
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL38B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL38B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2012-07-02 |
| Detector | ADSC QUANTUM 315 |
| Wavelength(s) | 1.000 |
| Spacegroup name | H 3 2 |
| Unit cell lengths | 103.948, 103.948, 364.474 |
| Unit cell angles | 90.00, 90.00, 120.00 |
Refinement procedure
| Resolution | 40.650 - 1.880 |
| R-factor | 0.1878 |
| Rwork | 0.187 |
| R-free | 0.20820 |
| Structure solution method | SAD |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.035 |
| Data reduction software | XDS |
| Data scaling software | XDS |
| Phasing software | SHELXDE |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Inner shell | Outer shell | |
| Low resolution limit [Å] | 40.650 | 40.660 | 1.990 |
| High resolution limit [Å] | 1.880 | 5.590 | 1.880 |
| Rmerge | 0.055 | 0.028 | 0.346 |
| Rmeas | 0.065 | 0.033 | 0.406 |
| Number of reflections | 118006 | 3956 | 19174 |
| <I/σ(I)> | 15 | 35.77 | 3.71 |
| Completeness [%] | 98.7 | 87.2 | 99.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5 | 293 | 2.5 M sodium formate, 0.1 M sodium acetate |
