5AX2
Crystal structure of S.cerevisiae Kti11p
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SLS BEAMLINE X06DA |
| Synchrotron site | SLS |
| Beamline | X06DA |
| Temperature [K] | 100 |
| Detector technology | PIXEL |
| Collection date | 2014-10-18 |
| Detector | DECTRIS PILATUS 2M |
| Wavelength(s) | 1.078 |
| Spacegroup name | I 2 2 2 |
| Unit cell lengths | 40.875, 53.656, 84.196 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 45.000 - 2.400 |
| R-factor | 0.2344 |
| Rwork | 0.231 |
| R-free | 0.26170 |
| Structure solution method | SAD |
| RMSD bond length | 0.013 |
| RMSD bond angle | 1.840 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | REFMAC (5.6.0117) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 45.000 | 2.450 |
| High resolution limit [Å] | 2.400 | 2.400 |
| Rmerge | 0.172 | |
| Number of reflections | 3858 | |
| <I/σ(I)> | 21.4 | 2.6 |
| Completeness [%] | 95.9 | 68.6 |
| Redundancy | 4.9 | 2.2 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION | 5.5 | 288 | Sodium acetate, Cadmium chloride, PEG 4000 |
