5AWX
Crystal structure of Human PTPRZ D1 domain
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | ROTATING ANODE |
| Source details | RIGAKU MICROMAX-007 |
| Temperature [K] | 100 |
| Detector technology | IMAGE PLATE |
| Collection date | 2013-04-17 |
| Detector | RIGAKU RAXIS IV++ |
| Wavelength(s) | 1.5418 |
| Spacegroup name | P 21 21 21 |
| Unit cell lengths | 54.641, 72.245, 90.557 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 31.400 - 1.860 |
| R-factor | 0.18745 |
| Rwork | 0.186 |
| R-free | 0.22094 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 3qcb |
| RMSD bond length | 0.021 |
| RMSD bond angle | 1.977 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | AMoRE |
| Refinement software | REFMAC (5.8.0073) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 31.400 |
| High resolution limit [Å] | 1.860 |
| Rmerge | 0.039 |
| Number of reflections | 29084 |
| <I/σ(I)> | 45.9 |
| Completeness [%] | 99.5 |
| Redundancy | 7.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 8.5 | 277 | 10% (w/v) PEG8000, 20% (v/v) ethylene glycol, 30mM sodium fluoride, 30mM sodium bromide, 30mM sodium iodide |
