5AWW
Precise Resting State of Thermus thermophilus SecYEG
Experimental procedure
Experimental method | SINGLE WAVELENGTH |
Source type | SYNCHROTRON |
Source details | SPRING-8 BEAMLINE BL32XU |
Synchrotron site | SPring-8 |
Beamline | BL32XU |
Temperature [K] | 100 |
Detector technology | CCD |
Collection date | 2015-01-20 |
Detector | RAYONIX MX225HE |
Wavelength(s) | 1.0 |
Spacegroup name | I 2 2 2 |
Unit cell lengths | 105.493, 112.904, 140.002 |
Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
Resolution | 43.943 - 2.724 |
R-factor | 0.2234 |
Rwork | 0.219 |
R-free | 0.26830 |
Structure solution method | MOLECULAR REPLACEMENT |
Starting model (for MR) | 2zjs |
RMSD bond length | 0.003 |
RMSD bond angle | 0.623 |
Data scaling software | SCALEPACK |
Phasing software | PHASER |
Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
Overall | |
Low resolution limit [Å] | 50.000 |
High resolution limit [Å] | 2.700 |
Number of reflections | 21452 |
<I/σ(I)> | 10.5 |
Completeness [%] | 94.5 |
Redundancy | 4.7 |
Crystallization Conditions
crystal ID | method | pH | temperature | details |
1 | LIPIDIC CUBIC PHASE | 6.5 | 293 | PEG 500MME, MgSO4, Na2SO4 |