5AWR
Crystal structure of the SGIP1 mu homology domain in the P4212 space group
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL26B1 |
| Synchrotron site | SPring-8 |
| Beamline | BL26B1 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-11-27 |
| Detector | RIGAKU SATURN A200 |
| Wavelength(s) | 0.97912 |
| Spacegroup name | P 4 21 2 |
| Unit cell lengths | 109.826, 109.826, 79.492 |
| Unit cell angles | 90.00, 90.00, 90.00 |
Refinement procedure
| Resolution | 38.829 - 2.502 |
| R-factor | 0.2014 |
| Rwork | 0.199 |
| R-free | 0.24730 |
| Structure solution method | SAD |
| RMSD bond length | 0.008 |
| RMSD bond angle | 1.140 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.8.2_1309)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 2.500 |
| Number of reflections | 17337 |
| <I/σ(I)> | 25.7 |
| Completeness [%] | 99.9 |
| Redundancy | 32.4 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, HANGING DROP | 5.1 | 293 | PEG 3350, zinc acetate, sodium acetate, sodium iodide |
