5AWN
Crystal structure of Human anti-HIV-1 broadly neutralizing antibody 3BC176 Fab
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | ALS BEAMLINE 4.2.2 |
| Synchrotron site | ALS |
| Beamline | 4.2.2 |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2013-10-18 |
| Detector | NOIR-1 |
| Wavelength(s) | 1.0 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 41.732, 60.955, 87.239 |
| Unit cell angles | 90.00, 95.39, 90.00 |
Refinement procedure
| Resolution | 49.894 - 1.887 |
| R-factor | 0.1797 |
| Rwork | 0.177 |
| R-free | 0.22300 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 5cck |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.148 |
| Data scaling software | HKL-2000 |
| Phasing software | PHASER |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | |
| Low resolution limit [Å] | 50.000 |
| High resolution limit [Å] | 1.887 |
| Number of reflections | 35026 |
| <I/σ(I)> | 16.7 |
| Completeness [%] | 99.7 |
| Redundancy | 3.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 5.6 | 298 | 0.1 M sodium citrate pH 5.6, 20% 2-propanol, 20% PEG 4000 |
