5AWG
Crystal structure of Hg-bound SufB-SufC-SufD complex from Escherichia coli
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL44XU |
| Synchrotron site | SPring-8 |
| Beamline | BL44XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2014-06-28 |
| Detector | RAYONIX MX300HE |
| Wavelength(s) | 0.950 |
| Spacegroup name | P 1 21 1 |
| Unit cell lengths | 119.848, 139.379, 124.414 |
| Unit cell angles | 90.00, 113.55, 90.00 |
Refinement procedure
| Resolution | 43.942 - 4.278 |
| R-factor | 0.2973 |
| Rwork | 0.295 |
| R-free | 0.34040 |
| Structure solution method | MIR |
| RMSD bond length | 0.007 |
| RMSD bond angle | 1.672 |
| Data reduction software | HKL-2000 |
| Data scaling software | HKL-2000 |
| Phasing software | PHENIX |
| Refinement software | PHENIX ((phenix.refine: 1.9_1692)) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 4.450 |
| High resolution limit [Å] | 4.278 | 4.300 |
| Rmerge | 0.127 | 0.295 |
| Number of reflections | 50117 | |
| <I/σ(I)> | 6.6 | 5.2 |
| Completeness [%] | 99.3 | 99.9 |
| Redundancy | 5.6 | 5.6 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | VAPOR DIFFUSION, SITTING DROP | 277 | pentaerythritol propoxylate (5/4 PO/OH), sodium citrate, potassium chloride |
