5AW8
Kinetics by X-ray crystallography: E2.MgF42-.2RB+ crystal
Experimental procedure
| Experimental method | SINGLE WAVELENGTH |
| Source type | SYNCHROTRON |
| Source details | SPRING-8 BEAMLINE BL41XU |
| Synchrotron site | SPring-8 |
| Beamline | BL41XU |
| Temperature [K] | 100 |
| Detector technology | CCD |
| Collection date | 2009-05-16 |
| Detector | RAYONIX MX225HE |
| Wavelength(s) | 0.8130 |
| Spacegroup name | C 1 2 1 |
| Unit cell lengths | 222.122, 50.706, 163.256 |
| Unit cell angles | 90.00, 104.85, 90.00 |
Refinement procedure
| Resolution | 14.990 - 2.800 |
| R-factor | 0.251 |
| Rwork | 0.251 |
| R-free | 0.25700 |
| Structure solution method | MOLECULAR REPLACEMENT |
| Starting model (for MR) | 2zxe |
| RMSD bond length | 0.009 |
| RMSD bond angle | 1.100 |
| Data reduction software | DENZO |
| Data scaling software | SCALEPACK |
| Phasing software | CNS (1.2) |
| Refinement software | CNS (1.2) |
Data quality characteristics
| Overall | Outer shell | |
| Low resolution limit [Å] | 50.000 | 2.860 |
| High resolution limit [Å] | 2.800 | 2.800 |
| Number of reflections | 42895 | |
| <I/σ(I)> | 19.5 | |
| Completeness [%] | 97.2 | |
| Redundancy | 5.7 |
Crystallization Conditions
| crystal ID | method | pH | temperature | details |
| 1 | MICRODIALYSIS | 7 | 298 | PEG 3000, MPD, potassium acetate, rubidium acetate, magnesium chloride, potassium fluoride, MES/TRIS |
